Table 4.
KD values for human FcγRs determined by SPR
| KD (μM) | FcγRI (×10−3) | FcγRIIaH131 | FcγRIIaR131 | FcγRIIb | FcγRIIIaF158 | FcγRIIIaV158 |
|---|---|---|---|---|---|---|
| wt-IgG1 | 0.21 ± 0.02 | 0.22 ± 0.06 | 1.16 ± 0.25 | 2.64 ± 0.39 | 1.17 ± 0.23 | 0.17 ± 0.04 |
| DHS-IgG1 | 0.28 ± 0.02 | 0.27 ± 0.04 | 0.95 ± 0.02 | 2.91 ± 0.54 | 0.48 ± 0.07 | 0.18 ± 0.03 |
| YTE-IgG | 0.29 ± 0.02 | 0.62 ± 0.18 | 3.20 ± 0.26 | 6.24 ± 0.10 | 4.41 ± 0.93 | 0.82 ± 0.11 |
| LS-IgG1 | 0.58 ± 0.12 | 0.41 ± 0.30 | 1.21 ± 0.02 | 2.25 ± 0.03 | 0.64 ± 0.09 | 0.52 ± 0.09 |
All IgG variants analyzed with monomeric Fc receptors and their kinetic values were analyzed by the equivalent binding model. Data are presented as mean ± standard deviation from triplicate experiments