Figure 5.

Accumulation of intra- and extracellular H₂O₂ after salt stress in WT and transgenic plants (S16-S-4) leaves. (A) Intracellular hydrogen peroxide was determined using confocal scanning microscopy followed by incubation with BES-H₂O₂-Ac. Images of H₂O₂ stained with BES-So-Am (green) and chlorophyll autofluorescence (red). (B) Extracellular hydrogen peroxide was determined using confocal scanning microscopy by incubation with BES-H₂O₂. Images of the H₂O₂ stained with BES-H₂O₂ (green) and chlorophyll autofluorescence (red). (C, D) Green fluorescence signals for intracellular H₂O₂ (C) and extracellular H₂O₂ (D) were quantified by ImageJ. The photographs represented (A, B) are from one representative experiment with three leaves after verifying the reproducibility of the results in three experiments. Data (C, D) were generated from 10 cells in one representative experiment after verifying the reproducibility of the results in three experiments. An asterisk indicates a significant difference between WT and transgenic plants (**P < 0.01).