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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Neurobiol Dis. 2019 Jul 23;132:104541. doi: 10.1016/j.nbd.2019.104541

Figure 2. Chronic insulin treatment (IR) and reduced insulin- and IGF-I-mediated decrease in APP phosphorylation.

Figure 2.

eCN were treated with 50 nM insulin for 24 h to model IR and then treated with 20 nM insulin or IGF-I for 2 h (A). Akt (B) and ERK (C) phosphorylation were analyzed against the total protein. (D) APP phosphorylation was analyzed by Western blotting and (E) measured against total APP level. The numbers inside the bars indicate the % changes compared to no treatment. Results are shown mean ± SEM by Student t-test.