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. Author manuscript; available in PMC: 2020 Nov 5.
Published in final edited form as: Cell Metab. 2019 Aug 29;30(5):877–889.e7. doi: 10.1016/j.cmet.2019.08.001

Figure 3. Alcohol-induced alteration of methionine cycle and transsulfuration pathway in the liver.

Figure 3.

(A) Graphical representation of transsulfuration pathway and xCT-mediated glutathione biosynthesis.

(B) Concentrations of methionine, SAM, SAH, homocysteine, cystathionine, cysteine, and GSH in liver tissue and serum homocysteine levels from pair-fed (n = 4) and EtOH-fed (n = 6) mice for 8 weeks.

(C and D) in situ closed liver perfusion system (C) were used for concentration measurements of methionine, SAM, SAH, homocysteine, cystathionine, cysteine, and GSH in WT mouse livers (n = 3/group) by circulating media containing 50 mM ethanol, and medium levels of ALT and glutamate (D).

(E) in vitro co-culturing HEPs with HSCs in the presence of ethanol plus sulfasalazine (SSZ) (3 replicates).

(F-H) Concentration of glutamate and cystine in media (n = 3/each) (F), and relative mRNA expression of Daglb in co-cultured HSCs (G) and those of Cnr1, Srebf1, and Fasn in HEPs (H).

Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.

See also Figure S2.