KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| β-actin (clone AC-74) | Sigma-Aldrich | Cat#: A5316; RRID: AB_476743 |
| p-AMPK (clone 40H9) | Cell Signaling Technology | Cat#:2535; RRID: AB_331250 |
| AMPK | Cell Signaling Technology | Cat#:2532; RRID: AB_330331 |
| FASN | Cell Signaling Technology | Cat#:3189; RRID: AB_2100798 |
| DAGLβ (D4P7C) | Cell Signaling Technology | Cat#:12574; RRID: AB_2797959 |
| p-p42/44 MAPK | Cell Signaling Technology | Cat#:9101; RRID: AB_331646 |
| ALDH4A1 (672) | Santa Cruz Biotechnology | Cat#:sc-100499; RRID: AB_2224476 |
| CYP2E1 | Millipore | Cat#:AB1252; RRID: AB_90517 |
| xCT | Thermo Fisher Scientific | Cat#:PA1–16893; RRID: AB_2286208 |
| GCLC | Thermo Fisher Scientific | Cat#:PA5–16581; RRID: AB_10977936 |
| NRF2 | Abcam | Cat#:ab31163; RRID: AB_881705 |
| mGluR5 | Abcam | Cat#:ab76316; RRID: AB_1523944 |
| GS | Abcam | Cat#: ab73593; RRID: AB_2247588 |
| GLS2 | Abcam | Cat#; ab113509; RRID: AB_10866157 |
| Anti-rabbit IgG, HRP-linked Antibody | Cell Signaling Technology | Cat#:7074; RRID: AB_2099233 |
| Anti-mouse IgG, HRP-linked Antibody | Thermo Fisher Scientific | Cat#:31430; RRID: AB_228307 |
| Anti-rabbit IgG H&L (Alexa Fluor® 488) | Abcam | Cat#:ab150061; RRID: AB_2571722 |
| Anti-rabbit IgG H&L (Alexa Fluor® 594) | Abcam | Cat#:ab150064; RRID: AB_2734146 |
| Anti-mouse IgG H&L (Alexa Fluor® 594) | Abcam | Cat#:ab150108; RRID: AB_2732073 |
| CD16/CD32 (Clone 2.4G2) | BD Biosciences | Cat#:553141, RRID: AB_394656 |
| CD45 (Clone 30-F11)-eFluor 450 | Thermo Fisher Scientific | Cat#: 48-0451-80; RRID: AB_1518807 |
| F4/80 (Clone BM8)-FITC | Thermo Fisher Scientific | Cat#: 11-4801-82; RRID: AB_2637191 |
| CD11b (Clone M1/70)-APC | BD Biosciences | Cat#: 553312; RRID: AB_398535 |
| Anti-mGluR5 Antibody (Alexa Fluor® 647) | Abcam | Cat#:ab196929 |
| Rabbit IgG - Isotype Control (Alexa Fluor® 647) | Abcam | Cat#:ab199093 |
| Bacterial and Virus Strains | ||
| DH5α Competent Cells | Invitrogen/Fisher Scientific | LS18265017 |
| pAAV-U6-sgRNA(shRNA)-CMV-GFP | Addgene | Cat#:85451 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Acrylamide | Bio-Rad | Cat#161–0156 |
| Blotting-Grade Blocker | Bio-Rad | Cat#: 170–6404 |
| Bradford reagent | Sigma-Aldrich | Cat#:B6916 |
| BSA | Sigma-Aldrich | Cat#:A9647 |
| CHPG | Tocris | Cat#:1049 |
| Collagenase type 1 | Worthington | Cat#:LS004197 |
| CTEP (RO4956371) | APExBIO | Cat#:B1633 |
| DAPI | Abcam | Cat#:ab104139 |
| DHPG | Tocris | Cat#:0805 |
| Dimethyl sulfoxide (DMSO) | Sigma-Aldrich | Cat#:D2650 |
| High glucose Dulbecco’s modified Eagle’s medium (DMEM) | Welgene | Cat#:LM001–05 |
| Histoacryl® | B Braun | NA |
| Low glucose DMEM | Welgene | Cat#:LM001–11 |
| Ethanol | Millipore | Cat#: 100983 |
| Fetal Bovine Serum (FBS) | Welgene | Cat#:S101–01 |
| KT172 | Sigma-Aldrich | Cat#:SML1688 |
| L-glutamic acid, monosodium salt, low toxin | Sigma-Aldrich | Cat#:RES5063G-A701X |
| MPEP hydrochloride | Tocris | Cat#:1212 |
| Optiprep™ density gradient medium | Sigma-Aldrich | Cat#:D1556 |
| Penicillin and streptomycin | Thermo Fisher Scientific | Cat#: 15240062 |
| Percoll® | Sigma-Aldrich | Cat#:P1644 |
| Protease and phosphatase inhibitor cocktail | Thermo Fisher Scientific | Cat#:78440 |
| Rimonabant hydrochloride | Tocris | Cat#0923 |
| Rosewell Park Memorial Institute (RPMI)-1640 | Welgene | Cat#:LM011–01 |
| Sapl | New England BioLabs | Cat#:R0569S |
| Sulfasalazine | Sigma-Aldrich | Cat#:S0883 |
| Triton X-100 | Sigma-Aldrich | Cat#:T9284 |
| Tween 20 | Sigma-Aldrich | Cat#:P1379 |
| TRIzol reagent | Thermo Fisher Scientific | Cat#: 15596–018 |
| Critical Commercial Assays | ||
| ALDH4A1 ELISA Kit | MyBioSource | Cat#:MBS9332194 |
| amfiRivert II cDNA Synthesis Master Mix | GenDEPOT | Cat#:R5500–100 |
| DAB peroxidase substrate | Vector Laboratories | Cat#:SK-4100 |
| ECL western blotting substrate | Thermo Fisher Scientific | Cat#:34095 |
| ImmPRESS HRP Anti-Rabbit IgG Polymer Detection Kit | Vector Laboratories | Cat#:MP-7401 |
| ReverTra Ace® qPCR RT Master Mix with gDNA Remover | Toyobo | Cat#:FSQ-301 |
| SYBR Green Realtime PCR Master Mix | Toyobo | Cat#:QPK-201 |
| Deposited Data | ||
| RNA-seq analysis of pair- and ethanol-fed mouse liver tissue | NCBI BioProject | PRJNA494040 |
| RNA-seq analysis of pair- and ethanol-fed mouse liver tissue | NCBI Gene Expression Omnibus | GEO: GSE97234 |
| Gene microarray of alcoholic hepatitis patient liver tissue | NCBI Gene Expression Omnibus | GEO:GSE28619 |
| Experimental Models: Cell Lines | ||
| Human: HEK293T cells | ATCC | CRL-3216 |
| Human: Hep3B cells | ATCC | HB-8064 |
| Human: HepG2 cells | ATCC | HB-8065 |
| Mouse: Neuro-2a cells | ATCC | CCL-131 |
| Experimental Models: Organisms/Strains | ||
| Cyp2e1 knock-out mice | Jackson Laboratories | #002910 (provided by Dr. Bin Gao) |
| Grm5 knock-out mice | Jackson Laboratories | #003121 |
| Slc7a11 tm1a mice | EMMA mouse repository | #EM:10001 |
| Albumin-Cre mice | Jackson Laboratories | #003574 |
| C57BL/6J | Jackson Laboratories | C57BL/6J |
| Oligonucleotides | ||
| primers for qPCR | Bioneer | See Table S3 |
| control shRNA | Bionner | See STAR★METHODS |
| Slc7a11 shRNA | Bioneer | See STAR★METHODS |
| Software and Algorithms | ||
| FlowJo, Version 10 | FlowJo | www.flowjo.com |
| Ingenuity Pathway Analysis | QIAGEN | www.ingenuity.com |
| GraphPad Prism version 7 | Prism | www.graphpad.com |
| Other | ||
| Lieber-DeCarli ethanol liquid diet | Dyets | Cat#:710260 |