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Method of DFGL (A) and labeling reaction on N-glycans (B) and O-glycans (C). In all cases, existing terminal sialic acids can be removed by neuraminidase treatment and replaced with fluorophore-conjugated sialic acids. Neu means neuraminidase; STs, sialyltransferases; CMP, cytidine monophosphate. Monosaccharide symbols follow the Symbol Nomenclature for Glycans system (PMID 26543186, Glycobiology 25: 1323–1324, 2015) details at NCBI.
