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. 2019 Sep 26;11(11):e10697. doi: 10.15252/emmm.201910697

Figure EV2. Validation of the lineage tracing system in Co‐mT/mG and DKO*‐mT/mG mice (related to Fig 2).

Figure EV2

  • A
    Whole mount of ear skin from co‐mT/mG mice before (VEHICLE) and after tamoxifen at day 15. White arrows represent isolated spots of GFP in vehicle skin.
  • B
    Immunofluorescence images of Co‐mT/mG ear skin sections and DKO*‐mT/mG at day 15 after tamoxifen treatment, co‐stained with K5 (white). Yellow dotted line separates epidermis and dermis.
  • C
    Immunofluorescence images of c‐Jun and JunB staining in DKO*‐mT/mG at day 15 after tamoxifen treatment showing GFP+ keratinocytes are negative for c‐Jun and JunB expression. GFP (green), Tomato (red), JunB and c‐Jun (white), DAPI (blue). Yellow dotted line separates epidermis and dermis.
  • D
    Representative images of whole mounts of ear skin from DKO*‐mT/mG at day 0 after tamoxifen treatment in two views, from the epidermis and from the dermis, to distinguish the expression of GFP+ epidermal cell into the IFE and HFs.