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Confocal immunofluorescent imaging of BAMs. BAMs generated from BSC, BSC + Hb, or BSC + Mb (3 mg/mL for both heme proteins) were stained after eight days of differentiation for DAPI, actin cytoskeleton (Phalloidin), and Troponin T (CT3), a marker of myogenesis. Images show multinucleated myotube formation in BSC and BSC + Mb constructs, though not in BSC + Hb constructs. Scale bars are 200 µm.
