Table 1.
Validation parameters for the methods used in the determination of coccidiostats in feed, muscle and eggs for residue control in Italy.
| Analyte | Matrix | LOD (µg/kg) | LOQ (µg/kg) | CV Repeatability (%) | CV Reproducibility (%) | Apparent Recovery Range (%) | Relative Expanded Uncertainty (%) | CCα (µg/kg) | CCβ (µg/kg) |
|---|---|---|---|---|---|---|---|---|---|
| NIC | Feed | 3 | 5 | 4.7 | 5.7 | 87–119 | 20.7 | ||
| Eggs | 1 | 1 | 3 | 4.7 | 83–115 | 323 | 348 | ||
| Poultry | 1 | 1 | 2.1 | 5.4 | 86–115 | 4354 | 4740 | ||
| Other species | 1 | 1 | 2.1 | 5.4 | 86–115 | 54 | 59 | ||
| ROB | Feed | 3 | 5 | 3.6 | 5.4 | 87–119 | 20.4 | ||
| Eggs | 1 | 1 | 4.8 | 6.9 | 83–115 | 28 | 31 | ||
| Poultry, rabbit | 1 | 1 | 5.5 | 9.1 | 86–115 | 230 a | 264 a | ||
| Other species | 1 | 1 | 5.5 | 9.1 | 86–115 | 5.7 | 6.6 | ||
| DIC | Feed | 3 | 5 | 5.1 | 6.3 | 87–119 | 21.4 | ||
| Eggs | 1 | 1 | 6.3 | 7.8 | 83–115 | 2.3 | 2.5 | ||
| Poultry | 1 | 1 | 4.2 | 8.5 | 86–115 | 570 | 649 | ||
| Rabbit | 1 | 1 | 4.2 | 8.5 | 86–115 | 171 | 195 | ||
| Other species | 1 | 1 | 4.2 | 8.5 | 86–115 | 5.7 | 6.5 | ||
| HFG | Feed | 3 | 5 | 6.5 | 9.7 | 87–119 | 25.9 | ||
| Eggs | 1 | 1 | 14 | 20.6 | 83–115 | 8 | 11 | ||
| Bovine | 1 | 1 | 9.5 | 14.4 | 86–115 | 12 | 15 | ||
| Other species | 1 | 1 | 9.5 | 14.4 | 86–115 | 3.7 | 4.6 | ||
| DEC | Feed | 3 | 5 | 3.4 | 5.3 | 87–119 | 20.3 | ||
| Eggs | 1 | 1 | 2.2 | 5.0 | 83–115 | 22 | 23 | ||
| Poultry | 1 | 1 | 2.3 | 10.7 | 86–115 | 588 | 691 | ||
| Other species | 1 | 1 | 2.3 | 10.7 | 86–115 | 24 | 28 | ||
| LAS | Feed | 3 | 5 | 5.2 | 6.3 | 87–119 | 21.4 | ||
| Eggs | 1 | 1 | 4.5 | 9.6 | 83–115 | 174 | 201 | ||
| Poultry | 1 | 1 | 4.5 | 9.4 | 86–115 | 69 | 80 | ||
| Bovine | 1 | 1 | 4.5 | 9.4 | 86–115 | 12 | 13 | ||
| Other species | 1 | 1 | 4.5 | 9.4 | 86–115 | 5.7 b | 6.6 b | ||
| MON | Feed | 3 | 5 | 4.4 | 6.9 | 87–119 | 22.1 | ||
| Eggs | 1 | 1 | 4.3 | 6.2 | 83–115 | 2.2 c | 2.4 c | ||
| Bovine | 1 | 1 | 4 | 6 | 86–115 | 2.2 | 2.4 | ||
| Poultry | 1 | 1 | 4 | 6 | 86–115 | 8.8 c | 9.6 c | ||
| Other species | 1 | 1 | 4 | 6 | 86–115 | 2.2 c | 2.4 c | ||
| SAL | Feed | 3 | 5 | 4.5 | 6.2 | 87–119 | 21.3 | ||
| Eggs | 1 | 1 | 5.3 | 7 | 83–115 | 3.3 d | 3.7 d | ||
| Poultry | 1 | 1 | 3.5 | 6.1 | 86–115 | 16.5 d | 18.1 d | ||
| Other species | 1 | 1 | 3.5 | 6.1 | 86–115 | 2.2 d | 2.4 d | ||
| NAR | Feed | 3 | 5 | 4.9 | 7.2 | 87–119 | 22.6 | ||
| Eggs | 1 | 1 | 5.6 | 7.3 | 83–115 | 2.2 | 2.5 | ||
| Poultry | 1 | 1 | 3.7 | 60 | 86–115 | 55 | 60 | ||
| Other species | 1 | 1 | 3.7 | 60 | 86–115 | 5.5 | 6 | ||
| SEM | Feed | 3 | 5 | 7 | 9.6 | 87–119 | 25.8 | ||
| Eggs | 1 | 1 | 8.7 | 15.1 | 83–115 | 2.5 | 3.1 | ||
| Other species | 1 | 1 | 6.6 | 10.5 | 86–115 | 2.3 | 2.7 | ||
| MAD | Feed | 3 | 5 | 6.6 | 9.2 | 87–119 | 25.3 | ||
| Eggs | 1 | 1 | 11.6 | 17.2 | 83–115 | 15 | 20 | ||
| Poultry | 1 | 1 | 6.7 | 11.4 | 86–115 | 36 e | 43 e | ||
| Other species | 1 | 1 | 6.7 | 11.4 | 86–115 | 2.4 | 2.8 |
a ROB hydrochloride, b LAS sodium, c MON sodium, d SAL sodium, e MAD ammonium.