Skip to main content
. 2019 Oct 10;11(10):2419. doi: 10.3390/nu11102419

Figure 3.

Figure 3

Effect of 4-HD on myogenesis of myoblasts. (A) Differentiated C2C12 cells in the presence of 4-HD (1, 10, 100 pM) were immunostained with antibody to MHC (red) and 4′,6-diamidino-2-phenylindole (DAPI, blue). Multinucleated (>4 nuclei) and MHC-expressing myotubes were counted from randomly selected areas. Scale bar = 200 μm. # p < 0.005 vs. 0 nM. (B) Expression levels of MHC were determined by Western blotting analysis. (C) C2C12 myoblasts were treated with 4-HD (100 pM) during myogenesis period (D1, D2, D3) and levels of myogenic factors were measured by Western blotting analysis.