Figure 1.

Experimental design. Eggs from Rhode Island Red (RIR) and Australorp (AUS) hens, which had been fed mixed feed, were collected at 33 weeks of age (mixed feed). Feed was switched at 34 weeks of age to fermented feed. Eggs were again collected at 41 weeks of age (fermented feed). Five eggs were collected at each stage from each breed; egg traits and yolk and albumen metabolite traits were measured in the 20 eggs. These data were analyzed using two-way mixed design analysis of variance (ANOVA) with breed group as the between-subjects factor and feed group as the within-subject factor.