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. 2019 Sep 21;9(10):197. doi: 10.3390/metabo9100197

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Volcano-plot statistical untargeted metabolomics comparison of the three fermentation additives tested versus their corresponding MMK2 control medium condition: (A) when XAD-16 adsorptive polymeric resin was added; (B) when the DNMT inhibitor 5-azacytidine was added; (C) when the HDAC inhibitor valproic acid was added (n = 10; α < 0.01). In blue the pseudomolecular ions m/z < 500 Da, in orange pseudomolecular ions m/z between 1000 and 1500 Da and in green pseudomolecular ions m/z > 1500 Da. Highlighted in other colours are the pseudomolecular ions responsible for the induced antitumoural activity of the compounds (see Identification of Bioactive compounds Section). −Log 10 of t-test statistical p-value in y-axis vs. −log 2 of m/z ion masses areas ratios in x-axes.