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. 2019 Oct 11;10(10):339. doi: 10.3390/insects10100339

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Knockdown of domeless and PGRP-LC enhanced the sigma virus replication in flies. (A) Green fluorescent protein (GFP) transgenic flies were injected with dsgfp and then reared for 3 days. The fluorescence was quenched in dsgfp-injected flies, indicating successful knockdown of the target gene by dsRNA injection. (B) RT-qPCR analysis of domeless and PGRP-LC gene expression in dsRNA injected flies. (C) The RT-qPCR analysis of sigma virus copies in sigma-virus-infected domeless and PGRP-LC knockdown and untreated flies. (D) The survival rate of sigma-virus-infected flies in domeless and PGRP-LC-knockdown flies upon CO₂ exposure. (E,F) RT-qPCR analysis of sigma virus copies in sigma-virus-infected domeless and PGRP-LC -knockdown and untreated flies. We set the dsControl group as 100% expression. The mean and SD are shown. ** p < 0.005, one-sample t-test. All experiments were performed with three replicates.

Knockdown of domeless and PGRP-LC enhanced the sigma virus replication in flies. (A) Green fluorescent protein (GFP) transgenic flies were injected with dsgfp and then reared for 3 days. The fluorescence was quenched in dsgfp-injected flies, indicating successful knockdown of the target gene by dsRNA injection. (B) RT-qPCR analysis of domeless and PGRP-LC gene expression in dsRNA injected flies. (C) The RT-qPCR analysis of sigma virus copies in sigma-virus-infected domeless and PGRP-LC knockdown and untreated flies. (D) The survival rate of sigma-virus-infected flies in domeless and PGRP-LC-knockdown flies upon CO₂ exposure. (E,F) RT-qPCR analysis of sigma virus copies in sigma-virus-infected domeless and PGRP-LC -knockdown and untreated flies. We set the dsControl group as 100% expression. The mean and SD are shown. ** p < 0.005, one-sample t-test. All experiments were performed with three replicates.