Figure 7.
CLSM images of (A) MCF-7 cells treated with FITC-labeled NPs (1 mg/mL) for 20 h, in which EEA1 was tested with primary antibody against EEA1, (B) DsRed-Rab7 transfected MCF-7 cells treated with FITC-labeled NPs (1 mg/mL) for 20 h, (C) EGFP-Rab7 transfected MCF-7 cells treated with Lyso-Tracker Red probes for 1 h, (D) MCF-7 cells treated with FITC-labeled NPs (1 mg/mL) for 20 h followed by treated with Lyso-Tracker Red probes for 1 h. (A–D) MCF-7 cells were irradiated by NIR (808 nm, 1.5 W/cm2, 5 min) at 0.5 h post-treatment. Scale bar: 20 μm, NPs: FITC/NPs@PDA-PEG-Apt.