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P-gp expression and localization by immunofluorescence microscopy in parental Hep3B and resistant Hep3B/PTX cells. The phase-contrast, P-gp (green), and Hoechst 33258 nuclear counter stain (blue). High levels of P-gp protein were localized in the plasma membrane of resistant cell Hep3B/PTX. Images were acquired at 40X objective plus a 2X zoom in confocal microscope settings. Scale bars, 40 μm.
