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Apoptosis induction by PTX and Achillin either alone or in combination in Hep3B/PTX cells. (a) Effect of PTX 25 nM, Achillin 100 µM, and PTX 25 nM + Achillin 100 μM on mRNA expression levels of Bcl-2 and Bax in Hep3B/PTX cells. GADHP was used as an internal control. (b) Graphic representation of effect on caspases activity of PTX 25 nM, Achillin 100 µM, and PTX 25 nM + Achillin 100 μM in Hep3B/PTX cells. Achillin was applied 2 h before PTX.** p ≤ 0.05.
