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. 2019 Oct 17;17(10):587. doi: 10.3390/md17100587

Figure 2.

Figure 2

Tv1 is selectively cytotoxic against liver cancer cells. (a) Cytotoxicity of Tv1 in different cancer cells. Fourty-eight hour treatment of 500 nM Tv1 indicated selective cytotoxicity for mouse liver cancer (1MEA) cells with a significant cell death of 40%. (n = 5). Origin of cancer cell lines used are HeLa-cervical cancer, SKnSH-neuroblastoma cells, WPE1-prostate cancer, 1MEA-liver cancer. (b) Comparative cytotoxicity of Tv1 (500 nM) with anticancer drugs Doxorubucin (1 μM) and Sorafenib (1 μM), which are commercially available. Tv1 demonstrated selective cytotoxicity to liver cancer cells, with diminished activity on normal liver cells, while Sorafenib and Doxorubicin showed high cytotoxicity and non-selectively in both the cell lines (n = 3). (c) Flow cytometry using AnnexinV/PI staining resulted in significant apoptotic cell death by 16 hour Tv1 treatment. n = 3 * p < 0.01. No treat indicates cells not treated with Tv1. Results are presented as mean ± Standard deviation. Analysis of variance (ANOVA) was used for statistical comparison between control and various treated groups.