Diurnal activity, food consumption, and circadian gene expression in type 2 diabetic mice (T2D). 10-month-old db/db (red) and aged-matched controls, db/m (black). (A,B) All measurements were performed after a 48-h acclimation period followed by 48-h of data collection every 10 min. (A) Mean activity over the night period (19:00–07:00) and the day period (07:00–19:00). n = 8 per group. Two-way ANOVA. (*) indicate statistical significance at post-hoc multiple comparison test. (B) Food consumption during the dark period and the day. n = 8 per group. Two-way ANOVA. Asterisks indicate statistical significance at post-hoc multiple comparison test. (C) mRNA expression of Per-2 in colonic tissue (n = 3). Two-way ANOVA. (*) indicate statistical difference p < 0.05, and (#) p < 0.01 in post-hoc comparisons. (D) mRNA expression of Bmal-1 in colonic tissue. (n = 3). Two-way ANOVA. (*) indicate statistical difference p < 0.05, and (#) p < 0.01 in post-hoc comparisons.