Figure 1.

Nitrated tyrosine residues are present in food extracts and stomach content. (A) In protein extracts from soy (1 µg), hazelnut (1 µg), celery (1 µg), and wheat (0.33 µg), nitrated tyrosine could be detected by an anti-3-NT antibody in dot blot experiments assaying triplicates. Walnut extract (1 µg) was negative for 3-NT. (B) Proteins extracted from the stomach contents of six naïve mice (14 µg) were screened for nitrated tyrosine residues. In five of six mice nitrated tyrosine residues could be detected (M1, M3, M4, M5, and M6) whereas M2 remained negative as compared to the controls.