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. 2019 Oct 15;11(10):2463. doi: 10.3390/nu11102463

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Re-stimulation of spleen cells with BLGu indicates the influence of treatment on cytokine production in non-allergic mice. Isolated spleen cells from non-allergic mice fed with BLGu or BLGn were stimulated with BLGu to evaluate the cytokine production upon allergen encounter. Baseline levels were subtracted. Spleen cells of BLGn-gavaged mice produced significantly higher levels of (A) IFN-γ (Mann–Whitney-U-test) and (B) IL-10 (unpaired t-test) compared to group BLGu. (C) IL-4 remained mostly below the detection limit of the assay used and did not show any differences (Mann–Whitney-U-test). * p < 0.05; BLG, beta-lactoglobulin; BLGn, nitrated BLG; BLGu, untreated BLG.