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. 2019 Sep 29;17(10):560. doi: 10.3390/md17100560

Figure 4.

Figure 4

HPLC analyses of secondary metabolites. (a) Comparative HPLC analysis of the secondary metabolites of the wild type S. atratus SCSIO ZH16NS-80S (WT) with regulatory and transporter gene-complementation strains. (I) the wild type S. atratus SCSIO ZH16NS-80S; (II) Δatr1 mutant strain; (III) Δatr1::atr1, atr1-complementation mutant strain; (IV) Δatr2 mutant strain; (V) Δatr2::atr2, atr2-complementation mutant strain; (VI) Δatr29 mutant strain; (VII) Δatr29::atr29, atr29-complementation mutant strain; (VIII) Δatr30 mutant strain; (IX) Δatr30::atr30, atr30-complementation mutant strain; (X) Δatr32 mutant strain; (XI) Δatr32::atr32, atr32-complementation mutant strain; (XII) the wild type S. coelicolor M1154 strain and (XIII) mutant strain S. coelicolor M1154 with PAC-434C. (b) Comparative analysis of atratumycin yield between over-expression mutants and the wild type strain (** p < 0.01). The values are mean ± SD from three different experiments.