Table 1.
Transcriptional effects of BPA on gilthead sea bream testis. Data are reported as mean ± SEM. All data are normalized to the expression level of cyt-pla2 POST-CTRL fish with an arbitrarily assigned value of 1. Asterisk superscript (*) indicates significant differences between the POST-CTRL group and the treatment (one-way ANOVA, Dunnett’s multiple comparisons test, * (p < 0.05), ** (p < 0.01), **** (p < 0.0001)). Abbreviations: cnr1: endocannabinoid receptor type i, cnr2: endocannabinoid receptor type ii, trpv1: transient receptor potential cation channel subfamily v member 1, ppar: peroxisome proliferator-activated receptor, nape-pld: n-acyl phosphatidylethanolamine phospholipase d, faah: fatty acid amide hydrolase, daglα: diacylglycerol lipase alpha, cox-2: cyclooxigenase-2, abdh: α/β-hydrolase, cyt-pla2: cytosolic phospholipase a2, lepr: leptin receptor, lepa: leptin a, er: estrogen receptor, pr: progesterone receptor, ar: androgen receptor, lhr: luteinizing hormone receptor, fshr: follicle-stimulating hormone receptor, gnrhr: gonadotropin-releasing hormone receptor, 17β-hsd: 17β-hydroxysteroid dehydrogenase, 3β-hsd: 3β-hydroxysteroid dehydrogenase.
| Gene | POST-CTRL | BPA LOW | BPA HIGH |
|---|---|---|---|
| cnr1 | 0.38 ± 0.07 | 0.64 ± 0.10 * | 0.60 ± 0.06 |
| cnr2 | 0.35 ± 0.04 | 0.24 ± 0.04 | 0.63 ± 0.08 ** |
| ppar α | 3.33 ± 0.34 | 2.43 ± 0.22 | 2.49 ± 0.24 |
| ppar β | 6.41 ± 0.63 | 6.82 ± 0.80 | 6.86 ± 0.72 |
| ppar γ | 12.68 ± 1.69 | 16.14 ± 2.13 | 12.63 ± 1.52 |
| trpv1 | 0.24 ± 0.03 | 0.37 ± 0.03 * | 0.30 ± 0.03 |
| faah | 4.87 ± 0.49 | 6.81 ± 0.67 | 4.81 ± 0.62 |
| nape-pld | 22.71 ± 1.55 | 25.50 ± 2.50 | 19.29 ± 1.94 |
| abdh4 | 3.69 ± 0.35 | 2.52 ± 0.35* | 3.00 ± 0.19 |
| cyt-pla2 | 1.00 ± 0.12 | 0.94 ± 0.12 | 1.22 ± 0.15 |
| cox2 | 1.43 ± 0.18 | 1.13 ± 0.12 | 0.98 ± 0.12 |
| daglα | 4.39 ± 0.46 | 5.96 ± 0.64 | 4,17 ± 0.49 |
| abdh 6a | 4.42 ± 0.34 | 3.56 ± 0.35 | 4.00 ± 0.36 |
| abdh 12b | 2.56 ± 0.29 | 2.65 ± 0.33 | 2.54 ± 0.34 |
| lepr | 15.88 ± 2.80 | 51.49 ± 5.16 **** | 24.75 ± 4.78 |
| lepa | 0.30 ± 0.03 | 0.25 ± 0.03 | 0.37 ± 0.04 |
| era | 3.04 ± 0.30 | 4.23 ± 0.34 * | 4.07 ± 0.33 |
| erb | 11.89 ± 0.67 | 11.68 ± 1.13 | 15.33 ± 1.02 * |
| pr | 4.36 ± 0.60 | 5.67 ± 0.66 | 7.57 ± 0.65 ** |
| ar | 8.59 ± 0.78 | 7.28 ± 0.89 | 7.72 ± 0.67 |
| lhr | 0.56 ± 0.06 | 0.50 ± 0.09 | 0.61 ± 0.04 |
| fshr | 0.46 ± 0.10 | 0.63 ± 0.07 | 0.56 ± 0.07 |
| gnrhr | 5.36 ± 0.81 | 5.11 ± 0.49 | 8.44 ± 0.96 * |
| 17b-hsd | 5.20 ± 0.50 | 4.75 ± 0.53 | 4.85 ± 0.45 |
| 3b-hsd | 5.91 ± 0.59 | 6.80 ± 0.62 | 5.63 ± 0.76 |