Table 1.
Effect of HAuCl4 concentration on the characteristics of the GNP 1.
| HAuCl4 Concentration (mM) | DLS (Reaction Mixture) | DLS (Purified Particles) | Morphology by TEM | ||||
|---|---|---|---|---|---|---|---|
| Deff (nm) | PDI | ζ-Potential (mV) | Deff (nm) | PDI | ζ-Potential (mV) | ||
| 0.05 | 50 ± 1 | 0.53 ± 0.02 | −3.76 ± 0.11 | 77 ± 16 | 0.18 ± 0.04 | −30.23 ± 0.05 | Multi-sized spheres |
| 0.10 | 122 ± 1 | 0.34 ± 0.001 | −3.52 ± 0.03 | 62 ± 1 | 0.25 ± 0.01 | −28.13 ± 1.29 | Multi-sized spheres |
| 0.15 | 216 ± 1 | 0.23 ± 0.01 | −3.18 ± 0.01 | 79 ± 1 | 0.19 ± 0.01 | −28.70 ± 3.41 | Triangles, rods, spheres |
| 0.20 | 231 ± 1 | 0.22 ± 0.02 | −2.45 ± 0.02 | 87 ± 1 | 0.14 ± 0.03 | −27.47± 0.06 | Triangles, plates, spheres |
| 0.25 | 229 ± 2 | 0.23 ± 0.01 | −3.01 ± 0.02 | 72 ± 1 | 0.12 ± 0.02 | −37.73 ± 0.25 | Spheres, triangles |
1 Particles were synthesized by mixing equal volumes of F127 solution (final concentration 6.3 % w/w, pH 6.5) and HAuCl4 solutions of different concentrations, followed by incubation of the mixture for 2 h at 55 °C. Particles were purified from excess of the polymer by centrifugal filtration at 1500 rpm for 30 min at 4 °C (repeated three times). The purified nanoparticles were restored to original volume with distilled water (particles concentration remained the same before and after purification).