Table 4.
Characteristics of purified GNP synthesized using non-lyophilized and lyophilized F127 samples 1.
| F127 Batch | GNP Synthesized Using Non-Lyophilized F127 | GNP Synthesized Using Lyophilized F127 | ||
|---|---|---|---|---|
| Deff (nm) | PDI | Deff (nm) | PDI | |
| F127_1 | 62 ± 1 | 0.16 ± 0.01 | 47 ± 1 | 0.30 ± 0.004 |
| F127_2 | 157 ± 2 | 0.06 ± 0.02 | 70 ± 1 | 0.07 ± 0.01 |
| F127_3 | Not formed | Not formed | 57 ± 1 | 0.30 ± 0.04 |
| F127_3 (pH 6.5) | 108 ± 2 | 0.17 ± 0.003 | n/a | n/a |
| F127_3 (pH 11.6) | 39 ± 3 | 0.51 ± 0.12 | n/a | n/a |
1 The 12.6% stock solutions of F127 solutions were prepared by dissolving the copolymer in bidistilled water. These solutions were lyophilized and restored to the same volume by bidistilled water. GNP were synthesized by mixing equal volumes of non-lyophilized and lyophilized F127 solutions and HAuCl4 solution (final concentrations 6.3% w/w F127 and 0.25 mM HAuCl4) followed by incubation of the mixture for 2 h at 45 °C. Particles were purified as described in Table 1. The purified GNPs were restored to the original volume using bidistilled water (particles concentration remained the same before and after purification).