COMSOL simulation to show the impact of channel length and flow rate ratio (Rf) on the concentration-controlled process. To explore the impact of channel length: (a) Concentration distribution of inlets, middle site and outlets of microfluidic chips with lengths of 30, 40, 50, 100 mm. (b) Concentration distribution of cross sections at the distances of 0, 10, 20, 30 mm away from inlets. (c) Calculate the concentration distribution of cross sections at the distances of 0, 10, 20, 30 mm away from inlets in microfluidic chips of 30, 40, 50, 100 mm. To explore the impact of Rf: (d) Concentration distribution of channel inlets, middle site and outlets under the flow rate ratio of 0.5, 1, 2, 4. (e) Concentration distribution of the cross section at the distances of 0, 10, 20, 30 mm away from inlets. (f) Calculate the concentration distribution of cross section at the distances of 0, 10, 20, 30 mm away from inlets under flow rate ratio of 0.5, 1, 2, 4. Data are shown as mean± SEM, n > 50, * p < 0.05, ** p < 0.01, *** p < 0.001.