Table 1.
qPCR primers for a standard set of measured transcripts.
| Disease/Host | Target | Forward Primer | Reverse Primer | Reference |
|---|---|---|---|---|
| Lotmaria passim-specific | Lotmaria passim | AGTATGAGCAGTAGGTTTTATTATA | GCCAAACACCAATAACTGGTACT | [62] |
| Deformed wing virus- | DWV-A,-B | ACGCAACCCCAGGAAT | GTAGCTAATTTTACCCAATCTTTAAA | [63] |
| Nosemosis | Nosema ceranae | TATTGTAGAGAGGTGGGAGATT | GTCGCTATGATCGCTTGCC | [53] |
| Bacterial infection | Bacteria (all), including Serratia | AGAGTTTGATCCTGGCTCAG | CTGCTGCCTCCCGTAGGAGT | [8] |
| Reference gene (host) | Ribosomal protein S5a (Rps5a) | AATTATTTGGTCGCTGGAATTG | TAACGTCCAGCAGAATGTGGTA | [8] |
| Reference gene (host) | Actin related protein 1 (Arp1) | CCAAAGACCCAAGCTCCCTA | TGGCTTATTGGTTTATGTTTTTCGT | [8] |
| Immunity gene (host) | Hymenoptaecin (Hym) | CTCTTCTGTGCCGTTGCATA | GCGTCTCCTGTCATTCCATT | [64] |
| Age/nutrition/immunity (host) | Vitellogenin (Vg) | TCGACAACTGCGATCAAAGGA | TGGTCACCGACGATTGGATG | [8] |