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. 2019 Sep 24;11(10):2280. doi: 10.3390/nu11102280

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Binding of MUC5b from UWS of older and younger adults to oral epithelial cells. (A) Mean (+/−SEM) binding of MUC5b from UWS of older (n = 21) and younger (n = 29) adults to TR146/MUC1 cells. MUC5b was quantified by blotting with anti-MUC5b antibody. (B) Representative image of immuno-blot for MUC5b (>250 kDa) from UWS of older and younger adults, binding to TR146/MUC1 cells. + = binding of control UWS sample, − = negative control (no WS), WS = control WS sample run as a comparison. Beta actin (43 kDa) used as loading control. Difference in means between groups tested for statistical significance using student’s t test. p = < 0.05 * p = < 0.01 **.