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. 2019 Oct 17;9(10):1475. doi: 10.3390/nano9101475

Figure 1.

Figure 1

Intracellular reactive oxygen species (ROS) generation and lipid peroxidation in MNPs@SiO2(RITC)-treated hBM-MSCs. (a) Schematic showing MNPs@SiO2(RITC) composition. (b) Morphological analysis of non-treated control and MNPs@SiO2(RITC)-treated hBM-MSCs. Scale bar = 50 µm (c) Cell viability assay with hBM-MSCs treated with MNPs@SiO2(RITC) for 12 h. Evaluation of intracellular ROS generation using DCFH-DA for 12 h in HEK293 cells treated with (d) MNPs@SiO2(RITC) and (e) silica NPs. The non-oxidized DCFH-DA was used as the blank. (f) Evaluation of peroxidized lipids using ferrous thiocyanate. Ferrous thiocyanate was used as the blank. Data represent mean ± SD of three independent experiments. * p < 0.05 vs. non-treated control, # p < 0.05 for the comparison between 0.1 and 1.0 µg/µL MNPs@SiO2(RITC) or silica NP-treated cells. Data represent mean ± SD of three independent experiments.