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. 2019 Oct 3;11(10):2355. doi: 10.3390/nu11102355

Figure 2.

Figure 2

Antiviral activity of NS MIX. Vero cells were pre-treated with 0.4, 0.2, and 0.1 mg/mL of NS MIX for 1 h and the cells were then infected with Herpes simplex virus type 1 strain F (HSV-1) for 1 h at 37 °C under gentle shaking. After incubation, the inoculum was removed and the cells were covered with medium containing 0.8% methylcellulose in the presence of NS MIX at 0.4, 0.2, and 0.1 mg/mL, separately. After three days the cells were fixed and stained with crystal violet and the plaques were visualized with an inverted microscope. (A) Plaque reduction assay following the NS MIX treatment. (B) Plaque morphological change due to the NS MIX treatment. Results are the mean of three biological independent experiments ± SD for each dilution. (C) Antiviral activity of acyclovir was tested in Vero cells infected with HSV-1 and incubated with medium containing 0.8% methylcellulose in the presence of acyclovir at 1, 10, and 20 μM. The cells were stained with crystal violet for plaque counting and detection. Data are expressed as a mean (± SD) of at least three experiments and asterisks (**, ***) indicate the significance of p-values less than 0.01, and 0.001, respectively.