Figure 4.

The effects of the subfractions from fr. F (with different concentrations) on cell viability and NO production in RAW264.7 cells. RAW264.7 cells were treated with various concentrations of CO-EtOAc (5, 10, and 25 μg/mL) in the presence or absence of LPS (1 μg/mL) for 24 h. (A) Cell viability was measured using the WST-1 assay, and the (B) NO production in cell culture supernatants determined using the Griess assay. The results are expressed as means ± SD from three independent experiments. For cell viability: * indicates p < 0.05 when compared to the DMSO control group. For NO production: values with different letters indicate significant difference (p < 0.05).