The effects of sitoindoside I, amentoflavone, and protocatechuic acid on cell viability and NO production in RAW264.7 cells. RAW264.7 cells were treated with various concentrations of frs. F5b, F5e, and F5f, sitoindoside I (S), amentoflavone (A), and protocatechuic acid (P) (10 and 5 μg/mL) in the presence or absence of LPS (1 μg/mL) for 24 h. (A) Cell viability was measured using the WST-1 assay, and the (B) NO production in cell culture supernatants determined using the Griess assay. The results are expressed as means ± SD from three independent experiments. For NO production: values with different letters indicate significant difference (p < 0.05).