Figure 4.

Effect on the mitochondrial potential in amoeba treated with the IC₉₀ of 7PSTS for 24 h (A) compared with the negative control (B) (overlay channels). JC-1 dye remained in the cytoplasm in its monomeric form, green fluorescence, and indicates the collapse of mitochondrial potential (A). In negative control (B) JC-1 dye accumulates in the mitochondria of healthy cells as aggregates (red fluorescence). All images (20×) are representative of the population of treated amoeba, and are based on Live Cell Imaging Microscope EVOS FL Cell Imaging System.