Table. Primers used for RT-PCR.

Primer name^a	Sequence (5’–3’)	Location (nt 5'–3´)^b	Annealing temperature (°C)	Fragment length^c
NP_F1	CATGCCAyTATATCCTCAAGTCAG	1,591–1,614	57	923
NP_R1b	GCATATrGGGTTGCACCACT	2,557–2,538	57	923
NP_F2	GATTTATTACGATACAACGArAAyGG	1,673–1,698	55	774
NP_R2	TCTATGCCCTCCTCTTGGA	2,491–2,473	55	774
PM_F1	GCTACAATTGAAGGAATGATGGC	2,766–2,788	60	859
PM_R1	TTGGGGATGCGGTTGATCT	3,667–3,649	60	859
PM_F2	GTCCCAGTTGATGAGCTTAGAAG	2,826–2,848	55	759
PM_R2	ACTyGCTGTCTTCCGAACC	3,626–3,608	55	759
MF_F1	TCATCACCATCGTTrGCGAA	4,185–4,204	55	504
MF_R1	GTTATCAGTGGGTTGGATATTyGG	4,731–4,708	55	504
MF_F2	TGGTCATCTGGGTGTGAAATC	4,212–4,232	55	428
MF_R2	GAGCTTGAACTTTGTGAGTAATAGC	4,685–4,661	55	428

nt: nucleotide.

^a NP, PM, MF: name of fragment containing the corresponding NCR; F: forward; R: reverse; 1: primary reaction; 2: nested reaction.

^b MuVi/Gloucester.GBR/32.96/ (AF280799) was used as reference sequence.

^c Length excluding primer sequences is presented here.