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. 2019 Sep 20;11(10):2264. doi: 10.3390/nu11102264

Figure 1.

Figure 1

Differential macrophage response to different inflammatory cytokine stimuli. (A) Morphological and immunocytochemical images showing monocyte-derived macrophage (MDM) polarization to M1 or M2 functional phenotype using type 1 cytokine IFN−γ and lipopolysaccharide (LPS) or type 2 cytokine IL-4 and IL-13, respectively. (B) Increases in CD80+ M1 and CD206+ M2 MDMs were seen after treatment with IFN−γ /LPS or IL-4/IL-13, respectively, using flow-cytometry analysis. Green arrows point to the CD80+ M1 macrophage, while red arrows indicate CD206+ M2 macrophages. (C) Flow cytometry and morphology imaging of M1 and M2 cell sorting and isolation using the fluorescence-activated cell sorting (FACS) assay. (D) Compared with the H1299 cells treated with M1 conditioned medium, the H1299 cells cultured with M2 conditioned medium exhibited higher invasion ability at 18 h in matrigel study. APC: antigen-presenting cell.