Figure 3.

Local toxicity of ciprofloxacin delivered using PVP-foils. (a) The XTT assay was run with a skin biopsy previously treated for 20 h with: 0.9% NaCl (1); PVP foils prepared from solutions in ddH₂O (2); PVP foils prepared from solutions in 30% acetic acid (3); PVP foils loaded with 11 µg (4), 250 µg (5), and 500 µg (6) of ciprofloxacin; and 50 µg of PVP-coated silver nanoparticles (7). Means and standard deviations from three independent experiments are reported. (b,c) Picture collage of H&E stained sections after 9 days of tissue culture of (b) an untreated wound and (c) a wound treated with a PVP foil loaded with 250 µg ciprofloxacin. (d–g) Details from the centre (d,f) and the edges (e,g) of control (d,e) and treated (f,g) wounds. Arrows show a newly formed epithelial layer.