Figure 3.

Effects of oral administration of L. fermentum PS150^TM with different intervention times and doses on pentobarbital-induced sleep in mice. Mice were orally administrated with PBS, 10⁸, or 10⁹ CFU of PS150^TM for 14 consecutive days. Pentobarbital (50 mg/kg)-induced sleep tests were performed on experimental days 1, 4, 7, and 14, and the sleep latency (n = 8~10) (a), sleep duration (n = 8~10) (b), and recovery time (n = 5~7) (c) of mice were recorded. DIPH (20 mg/kg) was introduced 30 min before the test. Veh refers to the vehicle control group. Data are expressed as mean ± SEM (n = 5~10) and were analyzed by one-way ANOVA with Tukey’s post hoc test. * p <0.05, compared with the indicated groups.