Table 2.
Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) analysis of the expression of genes encoding adenosine receptors and nucleotidase in the basal forebrain and hypothalamus of mice.
| Gene Name | Basal Forebrain | Hypothalamus | ||||||
|---|---|---|---|---|---|---|---|---|
| Veh | PS150 | Veh + Caffeine | PS150 + Caffeine | Veh | PS150 | Veh + Caffeine | PS150 + Caffeine | |
| A1R | 1.00 ± 0.13 | 0.91 ± 0.06 | 0.86 ± 0.17 | 1.00 ± 0.15 | 1.00 ± 0.11 | 1.38 ± 0.19 * | 1.08 ± 0.18 | 1.03 ± 0.11 |
| A2AR | 1.00 ± 0.21 | 0.90 ± 0.46 | 1.50 ± 0.3 | 1.39 ± 0.28 | 1.00 ± 0.14 | 0.95 ± 0.23 | 0.88 ± 0.15 | 0.89 ± 0.22 |
| NT5e | 1.00 ± 0.15 | 0.83 ± 0.19 | 0.90 ± 0.15 | 0.97 ± 0.22 | 1.00 ± 0.06 | 1.02 ± 0.23 | 1.09 ± 0.17 | 1.12 ± 0.23 |
The experimental design is shown in Figure 4a; the expression of genes was analyzed in the basal forebrain (n = 4~5) and hypothalamus (n = 6~8) of mice. Data are expressed as mean ± SEM and were analyzed by one-way ANOVA with Tukey’s post hoc test. * p <0.05, compared with the Veh group.