Figure 1.

Dot-blot screening of the yellow mealworm protein extract (protein concentration of 15 mg mL⁻¹) by 20 sera from shrimp-allergic patients, revealed with an anti-IgE second antibody. Data not shown for the single nonreactive serum #21. Depending on the patient sera, the IgE cross-reacting antibodies interacted strongly (dark dot) or weakly (grey dot) with the immobilized protein extract. No data for sera from dust mite allergic patients were shown because most of them (11/13), gave negative results. C+ and C− correspond to the positive and negative controls (first IgE antibody omitted) performed with a shrimp protein extract, respectively.