Effects of resveratrol on skeletal muscle lipids, and also expression and phosphorylation of activated protein kinase (AMPK), acetyl-CoA carboxylase (ACC) and protein kinase B (Akt) in muscle tissue of GK and SD rats. Triglycerides (A), non-esterified fatty acids (B). Western blot immunodetection with antibodies to the indicated proteins, GAPDH expression used as loading control (C). Protein levels relative to GAPDH quantified by densitometry: Total AMPK (D) and phosphorylation (pAMPK) (E), total ACC (F) and phosphorylated fraction (pACC) (G), total Akt (H) and pAkt (I). Results are representative of at least two independent experiments. GKC—Goto-Kakizaki control rats, GKR—Goto-Kakizaki resveratrol-treated rats, SDC—Sprague–Dawley control rats, SDR—Sprague–Dawley resveratrol-treated rats. Results are expressed as the means ± SEM from 10 rats in each group. Values with different letters are significantly different at p < 0.05.