Fig. 7.

Relative expressions of miRNA-targeted genes. Relative expression of each gene was determined through qRT-PCR. a Relative expressions of the known miRNA-targeted genes. b Relative expressions of the novel miRNA-targeted genes. The x-axis shows the names of the miRNA-targeted genes analyzed in this study. The y-axis shows the Log₂ratio between the expression values from a TbCSV/TbCSB-infected sample versus its Mock sample. Three biological replicates were analyzed for each miRNA-targeted gene through qRT-PCR. Expression level of N. benthamiana UBC gene was used as the reference gene during qRT-PCR assays. Asterisks indicate statistically significant differences compared with Mock, “*” indicate significant difference (0.01 ≤ p < 0.05), “**” indicate extremely significant difference (P < 0.01)