Table 2.

Primers for amplification of diarrheic E. coli virulence genes by conventional PCR

	Target genes	Primers	Primer sequence	Product size	Reference
A	Eae	eae-F	TCAATGCAGTTCCGTTATCAGTT	482 bp	[20, 21]
		eae-R	GTAAAGTCCGTTACCCCAACCTG
	Bfp	bfp-F	GGAAGTCAAATTCATGGGGGTAT	298 bp	[21]
		bfp-R	GGAATCAGACGCAGACTGGTAGT
	stx 1	stx1-F	CAGTTAATGTGGTGGCGAAGG	348 bp	[22]
		stx1-R	CACCAGACAATGTAACCGCTG
	stx 2	stx2-F	ATCCTATTCCCGGGAGTTTACG	584 bp	[22]
		stx2-R	GCGTCATCGTATACACAGGAGC
B	Est	ST-F	ATTTTTCTTTCTGTATTGTCTT	190 bp	[19]
		ST-R	CACCCGGTACAAGCAGGATT
	Elt	LT-F	GGCGACAGATTATACCGTGC	440 bp	[19]
		LT-R	CGGTCTCTATATTCCCTGTT
C	Ipa	ipaH-F	CTCGGCACGTTTTAATAGTCTGG	933 bp	[21]
		ipaH-R	GTGGAGAGCTGAAGTTTCTCTGC
	Aat	pCVD432-F	CTGGCGAAAGACTGTATCAT	630 bp	[19]
		pCVD432-R	CAATGTATAGAAATCCGCTGTT
	daaC	daaC-F	CAGGTCATCCGGTCAGTCGG	212 bp	[19]
		daaC-R	CAATGCCACGTACAACCGGC