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Open column chromatographic fractionation of the active fraction Pg-YP-I-22C obtained from the ethanolic extract of P. guajava bark
| Fraction code | Mobile phasea | Weight (mg) | Performance (%) |
|---|---|---|---|
| 22CK001 | MeOH:Acetic acid 0.1% (10:90) | 77.5 | 7.8 |
| 22CK002 | MeOH:Acetic acid 0.1% (15:85) | 17.5 | 1.8 |
| 22CK003 | MeOH:Acetic acid 0.1% (20:80) | 22.0 | 2.2 |
| 22CK004 | MeOH:Acetic acid 0.1% (25:75) | 44.5 | 4.5 |
| 22CK005 | MeOH:Acetic acid 0.1% (25:75) | 27.9 | 2.8 |
a Stationary Phase: Sephadex G10. Ratio sample/Sephadex: 1:20
