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. 2019 Nov 7;19:73. doi: 10.1186/s12896-019-0571-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Multiplex to singleplex qPCR Ct comparison. Graphics showing the comparison of the Ct values for each target (actin, P35S, P3, and TNOS) obtained from the multiplex assay (in black) and from the singleplex assay (in pink). The error bars show results from three independent experiments. (N = 3, no significant differences between any pair of multiplex vs. singleplex Ct values for the same plasmid concentration and target, Student’s t test, P < 0.05)