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Specificity of the multiplex qPCR assay. Log copy number of each target (actin, P35S, P3, and TNOS) for organic (watercress and ORG Canola) or GM (GM canola) plants infected or not with CaMV. The expected results are presented in the bar above the graphic (+ indicates expected amplification, − indicates no expected amplification). The error bars show results from three independent experiments, and no error bars are shown for actin because all copy numbers were normalized to actin
