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. 2019 Nov 7;19:73. doi: 10.1186/s12896-019-0571-1

Table 1.

Primer table. Table with the primers and probes used in the qPCR assays

Target Primers Sequences Amplicon (bp) References
Actin = Plant reference gene Actin Forward 5′-CAAGCAGCATGAAGATCAAGGT-3’ 103 [24]
Reverse 5′-CACATCTGTTGGAAAGTGCTGAG-3’
Probe 5′-HEX-CCTCCAATCCAGACACTGTACTTYCTCTC-BHQ-3’
CaMV Promoter 35S P35S Forward 5′-CGTCTACAAAGCAAGTGGATTG-3’ 79 [25]
Reverse 5′-TCTTGCGAAGGATAGTGGGATT-3’
Probe 5′-FAM-TCTCCACTGACGTAAGGGATGACGCA-QSY-3′
CaMV gene P3 P3 Forward 5′-TGAAATCCTCAGTGACCAAAAATC-3′ 152 [23]
Reverse 5′-TACAAGGACAATCATTGATGAGC-3′
Probe 5′-ABY-AAGCCGTTGCAGCGAAAATCGTTAATGA-QSY-3’
A.tumefaciens nopaline synthase terminator TNOS Forward 5′-GTCTTGCGATGATTATCATATAATTTCTG-3’ 151 [24]
Reverse 5′-CGCTATATTTTGTTTTCTATCGCGT-3’
Probe 5′-JUN-AGATGGGTTTTTATGATTAGAGTCCCGCAA-QSY-3′