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Limit of Detection (LOD) of the multiplex qPCR assay. The LOD for each target (actin, P35S, P3, and TNOS) is the percentage of plant DNA containing the target of interest diluted in relevant background DNA (i.e., background not containing the target of interest, see Methods) at the lowest concentration in which the target was detectable; 10 samples were tested for each dilution, and in each case, all 10 were positive (detected) at the LOD
| LOD (%) | |
|---|---|
| actin | 1 |
| P35S | 0.001 |
| P3 | 0.01 |
| TNOS | 0.01 |
