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. 2019 Nov 6;19:279. doi: 10.1186/s12935-019-0996-6

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — The functional role of RAD52 in HCC cell lines. a Expression level of RAD52 in human HCC cell lines including SMCC-7721 (7721), MHCC 97H (97H), MHCC 97L (97L), Huh7, HepG2 (G2), HCC-LM3 (LM3), and BEL7404 (7404) measured by Western blotting. Compared with the highest expression level of RAD52 in 7404, expression of RAD52 in Huh7 was lowest among these cells (P < 0.05, quantified by ImageJ). b Western blotting and qRT-PCR were used to detect RAD52 expression in RAD52-overexpression (OE)-transfected Huh7 cells. c CCK-8 test suggested that RAD52-OE in Huh7 cells could promote cell proliferation (P < 0.05). d Scratch wound assay showed that RAD52-OE in Huh7 cells could promote cell migration