Fig. 6.

NOTCH2 negatively regulates EMT in NPC cells via the TRAF6/AKT signaling axis. a Western blots showing that knockdown of NOTCH2 activated TRAF6 and that overexpression of NOTCH2 had the opposite effects. *P < 0.05, Student’s t-test. b Western blots demonstrate the knockdown of TRAF6 expression. *P < 0.05, one-way ANOVA. c Representative images of cytoskeletal (red) and DAPI (blue) staining in the indicated cells. d Transwell invasion assays showing that shTRAF6 reduced shNOTCH2 cell invasion in vitro. *P < 0.05, one-way ANOVA. e Western blots showing that the knockdown of TRAF6 attenuated the activation of AKT signaling components, reduced EMT-related vimentin expression and increased E-cadherin expression in shNOTCH2 cells. *P < 0.05, one-way ANOVA. Scale bars = 50 μm