Fig. 2. Optical properties of F16s. The absorption (a) and normalized emission (b) spectra of F16s were measured in water with a concentration of 31 μM and 7.8 μM, respectively (legend applies to both a and b). (c) F16s' relative quantum yields. EtOH as the solvent, compared with rhodamine 6G (QY: 95%). (d) Colocalization of 5BMF (GFP channel) with mitochondrial specific probe MitoTracker® red (microscopy RFP channel). The Pearson's correlation coefficient (γ) for 3T3, H838 and T24 is 0.978, 0.924, and 0.929, respectively (calculated using ImageJ, JACoP plugin). 63× oil objective lens. White scale bar: 10 μm.
