Skip to main content
. 2019 Oct 28;25:8084–8094. doi: 10.12659/MSM.918416

Figure 3.

Figure 3

NEAT1 is targeted by miR-181a. (A) Complementary sequences of miR-181a on NETA1 mRNA 3′-UTR were obtained from publicly available algorithms. (B) miR-181a mimics were transfected into chondrocytes to achieve miR-181a overexpression. (C) Luciferase activity of a luciferase reporter plasmid containing NEAT1-WT 3′UTR or NEAT1-MUT 3′UTR co-transfected with miR-181a mimics. (D) The RIP assay was performed to confirm whether NEAT1 and miR-181a could directly bind to Ago2. (E) The endogenous miR-181a level in chondrocytes treated with si-NEAT1. Data are shown as the mean±SD of 3 replicates. * P<0.05, ** P<0.01 vs. NC. NC – negative control.